kevinyx@life.illinois.edu
523 Burrill Hall
Office: (217) 265-9448
Lab: (217) 333-4984
Fax: (217) 333-1133
Mail to:
Department of Molecular and Integrative Physiology
523 Burrill Hall, MC-114
407 S. Goodwin Ave
Urbana, IL 61801
Kevin Y Xiang
Assistant Professor of Molecular and Integrative Physiology
Education
B.Sc. Wuhan University, China
PhD. Oregon Health Sciences University
Postdoc. Stanford University
Teaching Interests
GPCR signaling transduction in brain and heart
Research in my lab focuses on the structural functions of adrenergic receptors, a family of G-protein coupled receptor (GPCRs) in two areas: heart failure and Alzheimer’s diseases. The goal is to identify novel therapeutic strategies and targets for a variety of clinical conditions such as heart failure and hypertension, as well as Alzheimer’s diseases via integrated approaches.
GPCRs are nature's most versatile biological sensors. They conduct the majority of transmembrane responses to hormones and neurotransmitters, and mediate the senses of pressure, sight, smell, and taste in peripheral tissues and regulate mood, sleep, and behaviors in CNS. We are using integrated approaches including single molecule study, living-cell imaging study, and transgenic animals to understand the fundamentals of GPCR signaling transduction, and their functional implication in tissues, as well as pathogenesis of heart failure and Alzheimer’s diseases. Our current research focuses on three different areas.
1. Characterizing the signaling complexes associated with adrenergic receptors using single molecule co immunoprecipitation, and studying subcellular signaling events (i.e. cAMP, PKA, ERK, Akt, and Ca2+ signaling) using FRET-based living-cell imaging during receptor activation as well as their implication in cellular function.
2. Adrenergic receptor signaling in Alzheimer’s diseases. We are examining the receptor signaling induced by amyloid Aβ peptide in neurons and its implication on synaptic function and memory with novel transgenic AD animal models.
3. Signaling crosstalk in regulation of neuronal and cardiac function in disease conditions such as hypertension and depression.
Representative Publications
Cervantes D, Crosby C, Xiang Y. (2009) Arrestin orchestrates cross-talk between GPCRs to modulate the spatiotemporal activation of ERK MAPK Circulation Research. (in press)
De Arcangelis V, Liu R, Soto D, Xiang Y. (2009) Differential binding of phosphodiesterase 4D isoforms to β2 adrenoceptor in cardiac myocytes" J. Biol. Chem. Published online on October 2, 2009
Liu R, Ramani B, Soto D, De Arcangelis V, Xiang Y. (2009) Agonist dose-dependent PKA and GRK phosphorylation regulate β2 adrenoceptor coupling to Gi proteins in cardiomyocytes J. Biol. Chem. Published online on August 25, 2009
Yum K, Na S, Xiang Y, Wang N, Yu M. (2009) Mechanochemical delivery and dynamic tracking of single fluorescent quantum dots in Living Cells. Nano letter 9(5):2193-8
Soto D, De Arcangelis V, Zhang J, Xiang Y. (2009) Dynamic PKA activities induced by adrenoceptors dictate signaling propagation for substrate phosphorylation and myocyte contraction Circulation Research. 27;104(6):770-9
Zhang, H.T., Whisler, L.R., Huang, Y., Xiang, Y., and J.M. O'Donnell. 2009. Postsynaptic alpha-2 Adrenergic Receptors are Critical for the Antidepressant-Like Effects of Desipramine on Behavior. Neuropsychopharmacology0 34(4):1067-77.
2008
De Arcangelis V, Soto D, Xiang Y. (2008) Phosphodiesterase 4 and phosphotase 2A differentially regulate cAMP/PKA signaling for cardiac myocyte contraction under stimulation of beta1 adrenergic receptor. Mol. Pharmacology. 74(5):1453-62
Wang Y, De Arcagelis V, Ramali, B, Jung YS, Xiang Y. (2008) Norepinephrine and epinephrine induced distinct 2 adrenoceptor signaling is dictated by GRK phosphorylation in cardiomyocytes. J Biol. Chem. 283(4):1799-1807 [Download]
2007
Shcherbakova OG, Hurt CM, Xiang Y, Dell'Acqua ML, Zhang Q, Tsien RW, Kobilka BK.(2007) Organization of beta-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes. J Cell Biol. 176(4):521-33. [Download]
Sun Y, Huang J, Xiang Y, Bastepe M, Juppner H, Kobilka BK, Zhang JJ, and Huang X. (2007) Dosage-dependent switch from G protein-coupled to G protein-independent signaling by a GPCR. The EMBO Journal. 26(1):53-64 [Download]
Wang Y, Lauffer B, Von Zastrow M, Kobilka B, Xiang Y. (2007) NSF Regulates {beta}2 Adrenoceptors Trafficking and Signaling in Cardiomyocytes. Mol. Pharmacology. 72(2):429-439 Download