Brian C. Freeman

bfreeman@life.illinois.edu

B605 CLSL
Office: (217) 244-2662
Lab: (217) 244-1065

Mail to: Dept. of Cell & Developmental Biology
University of Illinois
B107 CLSL
601 S. Goodwin Avenue
Urbana, IL 61801
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Brian C. Freeman

Professor of Cell and Developmental Biology
Alexander von Humboldt Scholar

Research Topics

Genomics, Protein Dynamics, Protein-Nucleic Acid Interactions, Proteomics, Regulation of Gene Expression

Education

B.S., University of Michigan (Microbiology)
Ph.D., Northwestern University (Biochemistry and Biophysics)
Postdoctorate, University of California-San Francisco

Teaching Interests

Molecular Chaperones; Transcription; Chromatin Remodeling

Physiological balance is maintained, in part, through the rapid and well-timed assembly and disassembly of biological complexes. The dynamic interplay between select factors, including but not limited to proteins and nucleic acids, facilitates an efficient and functional cell environment. In general, pathways are driven forward through cooperative interactions thereby providing important features—rapid and robust action with limited energy input. However, within multi-step pathways cooperative stable assemblies have drawbacks since each complex is recalcitrant to dissociation. Hence, transitions between functional complexes or termination of action would be slow unless catalyzed. Despite the commonality of this problem, the possible cellular mechanisms promoting disassembly-events are not well understood. The primary focus of our laboratory is to identify and characterize the machinery driving a dynamic protein environment.

The Freeman research team exploits two central nuclear processes, transcription and chromatin remodeling, as molecular paradigms for understanding how the action of any single system can impact homeostasis, as dysfunction of either pathway can lead to cell death or uncontrolled growth (i.e., cancer). Since both processes employ a number of factors with common binding specificities, a decline in pathway efficiency is highly plausible due to the misassembly of individual complexes or an impairment in the disassembly of the utilized structures. The fundamental properties of molecular chaperones (abundant proteins with promiscuous but weak binding activities) allow biological factors to avoid these challenges by cultivating a self-organizing environment that fosters rapid action. In essence, the ability of molecular chaperones to promote protein dynamics parallels the more established chaperone roles in protein folding in which a chaperone does not dictate the final folded structure (path direction) but rather helps the nascent chain (system) avoid off-pathway energy barriers that commonly occur in protein folding (multi-step) energy landscapes. Our end goal is to understand the mechanisms by which molecular chaperones foster a dynamic nuclear environment thereby ensuring homeostasis.

Awards

TUM Ambassador, Technische Universität München, 2015.
Friedrich Wilhelm Bessel Research Award, Alexander von Humboldt Foundation, 2010.
Honorary Hans Fischer Senior Fellow, TUM-IAS, 2010.
Educator of the year. Alumni Association, University of Illinois, 2009.
American Heart Association Fellow, 2000-2002.
Leukemia and Lymphoma Society Fellow, 1997-2000.
Leukemia Research Foundation Fellow, 1996-1997.

Representative Publications

Echtenkamp, F.J., Z. Gvozdenov, N.L. Adkins, Y. Zhang, M.A. Day-Lynch, S. Watanabe, C.L. Peterson and B.C. Freeman (2016) Hsp90 and p23 chaperones control chromatin architecture by maintaining the functional pool of the RSC remodeler. Mol. Cell, 64, 888-899. (ABSTRACT)

Zelin, E. and B.C. Freeman (2015) Lysine deacetylases regulate the heat shock response including the age-associated impairment of HSF1. J. Mol. Biol., 427, 1644-1654. (ABSTRACT)

Echtenkamp, F.J. and B.C. Freeman (2014) Molecular Chaperone-Mediated Nuclear Protein Dynamics. Curr. Prot. Pept. Sci., 15, 216-224. (ABSTRACT)

Zelin, E., Y. Zhang, O.A. Toogun, S. Zhong and B.C. Freeman (2012) The p23 Molecular Chaperone and GCN5 Acetylase Jointly Modulate Protein-DNA Dynamics and Open Chromatin Status. Mol. Cell, 48, 459-470. (ABSTRACT)

Echtenkamp, F.J. and B.C. Freeman (2012) Expanding the Cellular Molecular Chaperone Network through the Ubiquitous Cochaperones. BBA - Molecular Cell Research: Special Issue on Heat Shock Protein 90 (Hsp90), 45, 8624-8632. (ABSTRACT)

Echtenkamp, F.J., E. Zelin, E. Oxelmark, J.I. Woo, B.J. Andrews, M.J. Garabedian and B.C. Freeman (2011) Global Functional Map of the p23 Molecular Chaperone Reveals an Extensive Cellular Network. Mol. Cell, 43, 229-241. (ABSTRACT)

DeZwaan, D.C. and B.C. Freeman (2010) Hsp90 manages the ends. Trends Biochem. Sci., 7, 384-391. (ABSTRACT)

Complete Publications List