Ann M Nardulli

nardulli@life.illinois.edu

513 Burrill Hall
Office: (217) 244-5679
Lab: (217) 244-6143
Fax: (217) 333-1133

Mail to: Department of Molecular and Integrative Physiology
524 Burrill Hall
407 S. Goodwin Ave
Urbana, IL 61801
Video Interview

Ann M Nardulli

Professor of Molecular and Integrative Physiology

Research Topics

Chromatin Structure, Endocrinology, Protein-Nucleic Acid Interactions, Receptor Biochemistry, Regulation of Gene Expression, Reproductive Biology, Signal Transduction

Education

B.S. 1970 Northern Illinois University
M.S. 1984 University of Illinois
PhD. 1987 University of Illinois
Postdoc. 1987-92 University of Illinois

Teaching Interests

Regulation of estrogen-responsive genes; role of estrogen-induced oxidative stress and DNA repair proteins in mammary carcinogenesis and breast cancer progression; mechanisms by which estrogen promotes neuroprotection

The estrogen receptor mediates the effects of estrogens and antiestrogens in target cells by binding of the receptor to specific DNA sequences present in target genes. This interaction of the estrogen receptor with DNA plays a critical role in development and maintenance of reproductive, cardiovascular, neural, and skeletal cells. Although the receptor-DNA interaction is of paramount importance in the regulation of estrogen-responsive genes, the mechanism by which this interaction leads to changes in gene expression is not well understood.

Using highly sensitive molecular techniques, we are studying the expression of endogenous, estrogen-responsive genes in human breast cancer cells to define how chromatin structure plays a role in estrogen- and antiestrogen- regulated gene transcription. Understanding the mechanisms by which estrogen agonists and antagonists modulate gene expression is particularly important in light of the fact that these compounds are widely used in hormone replacement therapy and in breast cancer treatment and prevention.

We are also interested in examining the effect of estrogen on expression of oxidative stress and DNA repair proteins in the brain using brain slice cultures, primary neurons, and whole animals. These studies will help to define mechanisms by which estrogen alters protein expression in the brain and helps to protect the brain from ischemic injury as occurs during stroke.

Representative Publications

Rao AK, Dietrich AK, Ziegler YS, and Nardulli AM. 2011 17β-Estradiol-mediated increase in Cu/Zn superoxide dismutase expression in the brain: A mechanism to protect neurons from ischemia. J Steroid Biochem Mol Biol. In Press.

Schultz-Norton JR, Ziegler YS, Nardulli AM. 2011 ERα-associated protein networks. Trends Endocrinol Metab Apr;22(4):124-129.

Bonéy-Montoya J, Ziegler YS, Curtis CD, Montoya JA, Nardulli AM 2010 Long-range transcriptional control of progesterone receptor gene expression Mol Endocrinol 24:246-258.

Curtis CD, Thorngren DL, Nardulli AM 2010 Immunohistochemical analysis of oxidative stress and DNA repair proteins in normal mammary and breast cancer tissues. BMC Cancer 10:9.

Schultz-Norton JR, Ziegler YS, Likhite VS, Nardulli AM 2009 Isolation of proteins associated with the DNA-bound estrogen receptor alpha. In Methods in Molecular Biology Molecular Endocrinology, A Comprehensive Guide to Current Methodologies, Humana Press 590:209-221.

Curtis CD, Thorngren DL, Ziegler YS, Sarkeshik A, Yates JR, Nardulli AM 2009 Apurinic/apyrimidinic endonuclease 1 alters estrogen receptor activity and estrogen responsive gene expression. Mol Endocrinol 23:1346-1359.

Rao A, Ziegler Y, McLeod I, Yates J, Nardulli AM 2009 Thioredoxin and thioredoxin reductase influence estrogen receptor α mediated gene expression in human breast cancer cells. J Mol Endocrinol 43:251-261.

Curtis CD, Nardulli AM 2009 Using RNA interference to study protein function. Methods in Molecular Biology 505:187-204.

Schultz-Norton JR, Ziegler YS, Likhite VS, Nardulli AM 2009 Isolation of proteins associated with the DNA-bound estrogen receptor alpha. In Methods in Molecular Biology Molecular Endocrinology, A Comprehensive Guide to Current Methodologies, Humana Press 590:209-221.

Curtis CD, Thorngren DL, Ziegler YS, Sarkeshik A, Yates JR, Nardulli AM 2009 Apurinic/apyrimidinic endonuclease 1 alters estrogen receptor activity and estrogen responsive gene expression.Mol Endocrinol 23:1346-1359.

Rao A, Ziegler Y, McLeod I, Yates J, Nardulli AM 2009 Thioredoxin and thioredoxin reductase influence estrogen receptor α mediated gene expression in human breast cancer cells. J Mol Endocrinol 43:251-261. ciated with DNA-bound Estrogen Receptor BMC Mol Biol In Press

Complete Publications List