CDB Microscopy Facility

Policies and Procedures

The facility is located in room C501 CLSL building.

Hours of operation are 8 a.m. to 5 p.m. on weekdays, unless otherwise designated.

The microscopes are available for all trained campus users with active accounts.

Training is usually two sessions of two hours each. During the second session, before being given the password and access, new users ensure that all basic procedures are memorized and performed well, with due care to the equipment, especially to the objectives and all optical components.

Assistance optimizing preparation techniques for selected projects is available at a laboratory in Morrill Hall on the basis of individual arrangements. Collaborative projects developing computer vision tools for automated pattern recognition in microscopy are in progress. Ideas and suggestions for collaboration are greatly welcome.

Microscopes

Zeiss LSM 510.
Zeiss LSM 510
confocal

Confocal laser scanning microscope
Argon2 laser 458, 477, 488, 514 nm
HeNe1 laser 543 nm
NeNe2 laser 633 nm
Plan-Neofluar 10x/ 0.3
Plan-Neofluar 20x/ 0.5
Plan-Neofluar 40x/ 0.75
Plan-Neofluar 40x/ 1.3 Oil
Plan-Apochromat 63x/ 1.4 Oil DIC objective

Download microscope manual [PDF 10,170 KB]


OMX V2.
Applied Precision OMX V2 with DeltaVision

Super-resolution microscope information

OMX V2 Imaging platform including super resolution technology providing spatial resolution far better than the diffraction limited performance in a normal epi-fluorescent microscope. The platform also includes the integrated capability for multiple cameras that can expose simultaneously for extremely fast, high signal-to-noise, live cell, and multiple wavelength imaging applications.

Read a feature article about the OMX microscope.


Zeiss Axioplan 1.
Zeiss Axioplan 1
fluorescence

Universal microscope with DIC, phase contrast and fluorescence capabilities.
Plan-Neofluar 20x/0.5
Plan-Neofluar 40x/ 0.75
Plan-Neofluar 40x/ 1.3 Oil
Plan-Neofular 100x/ 1.3 Oil objective
Photometrics SenSys camera with KAF 1400 G2 chip, 6.8 µm pixel
Device control and image acquisition with IP Lab v. 3.6 for Macintosh

Download software manual [PDF 3.312 KB] IP Lab for Mac v. 3.6

Download software extention [PDF 2,772 KB] IP Lab for Mac v. 3.6 / device control

Download microscope manual [PDF 1,647 KB]


Zeiss Axiovert 200M.

Zeiss Axiovert 200M
inverted fluorescence

The microscope is being equipped with additional components and will be available for use soon.

Download manual [PDF 5,339 KB]


Leica MZFL III.

Leica MZFL III
stereo fluorescence

Stereomicroscope with separate beam path (TripleBeam™)
Excitation and barrier filters in one filter carrier
Light source 50W mercury-vapor burner
Zoom 12.5:1, 10 engageable steps
Zoom range 8x–100x, total magnifications 5x to 800x
Numerical aperture 0.125 with plano objective 1x
Field diameter 0.4 - 52,5 mm
Working distances 134 mm, 90 mm, 60 mm, 55 mm, 19 mm
Wide-field eyepieces 10x

Download manual [PDF 1,451 KB]