Ann M Nardulli
514 Burrill Hall
Office: (217) 244-5679
Lab: (217) 244-6143
Fax: (217) 333-1133
Mail to: Department of Molecular and Integrative Physiology
524 Burrill Hall
407 S. Goodwin Ave
Urbana, IL 61801
Professor of Molecular and Integrative Physiology
Chromatin Structure, Endocrinology, Protein-Nucleic Acid Interactions, Receptor Biochemistry, Regulation of Gene Expression, Reproductive Biology, Signal Transduction
B.S. 1970 Northern Illinois University
M.S. 1984 University of Illinois
PhD. 1987 University of Illinois
Postdoc. 1987-92 University of Illinois
Regulation of estrogen-responsive genes; role of estrogen-induced oxidative stress and DNA repair proteins in mammary carcinogenesis and breast cancer progression; mechanisms by which estrogen promotes neuroprotection
The estrogen receptor mediates the effects of estrogens and antiestrogens in target cells by binding of the receptor to specific DNA sequences present in target genes. This interaction of the estrogen receptor with DNA plays a critical role in development and maintenance of reproductive, cardiovascular, neural, and skeletal cells. Although the receptor-DNA interaction is of paramount importance in the regulation of estrogen-responsive genes, the mechanism by which this interaction leads to changes in gene expression is not well understood.
Using highly sensitive molecular techniques, we are studying the expression of endogenous, estrogen-responsive genes in human breast cancer cells to define how chromatin structure plays a role in estrogen- and antiestrogen- regulated gene transcription. Understanding the mechanisms by which estrogen agonists and antagonists modulate gene expression is particularly important in light of the fact that these compounds are widely used in hormone replacement therapy and in breast cancer treatment and prevention.
We are also interested in examining the effect of estrogen on expression of oxidative stress and DNA repair proteins in the brain using brain slice cultures, primary neurons, and whole animals. These studies will help to define mechanisms by which estrogen alters protein expression in the brain and helps to protect the brain from ischemic injury as occurs during stroke.
Ziegler YS, Moresco JJ, Tu PG, Yates JR 3rd, Nardulli AM. 2014 Plasma membrane proteomics of human breast cancer cell lines identifies potential targets for breast cancer diagnosis and treatment. PLoS One Jul 16;9(7):e102341. PMID: 25029196
Dietrich AK, Humphreys GI, Nardulli AM 2013 17β-estradiol increases expression of the oxidative stress response and DNA repair protein apurinic endonuclease (Ape1) in the cerebral cortex of female mice following hypoxia. J Steroid Biochem Mol Biol. 410-20. doi: 10.1016/j.jsbmb.2013.07.007.
Yuan L, Dietrich AK, Nardulli AM 2013 17β-Estradiol alters oxidative stress response protein expression and oxidative damage in the uterus. Mol Cell Endocrinol. 382(1):218-226. PMID: 24103313.
Shim J, Humphreys GI, Venkatesan BM, Munz JM, Zou X, Sathe C, Schulten K, Kosari F, Nardulli AM, Vasmatzis G, Bashir R 2013 Detection and quantification of methylation in DNA using solid-state nanopores. Sci Rep 3:1389. PMID: 23474808.
Schultz-Norton JR, Ziegler YS, Nardulli AM. ERα-associated protein networks. Trends Endocrinol Metab. 2011 Apr;22(4):124-9. PMID: 21371903.
Curtis CD, Thorngren DL, Nardulli AM 2010 Immunohistochemical analysis of oxidative stress and DNA repair proteins in normal mammary and breast cancer tissues. BMC Cancer 10(1):9. PMID:20064251, PMCID: PMC2830938
Curtis CD, Thorngren DL, Ziegler YS, Sarkeshik A, Yates JR, Nardulli AM 2009 Apurinic/apyrimidinic endonuclease 1 alters estrogen receptor activity and estrogen responsive gene expression.Mol Endocrinol 23:1346-1359.
Bonéy-Montoya J, Ziegler YS, Curtis CD, Montoya JA, Nardulli AM 2010 Long-range transcriptional control of progesterone receptor gene expression Mol Endocrinol 24:246-258. PMID:19952285, PMCID: PMC2817601