James A Imlay
Professor of Microbiology
Associate Director, MCB Graduate Program
Enzymology, Microbial Physiology
B.S. (Chemistry, English), Duke University, 1981
Ph.D. (Biochemistry), University of California, Berkeley, 1987
Postdoctoral (Biochemistry), Duke University, 1987-1992
Molecular mechanisms of oxidative damage; cellular defenses against oxidants; obligate anaerobiosis
Oxygen is a reactive chemical, and its gradual accumulation in the environment must have been a problem for early life forms. For kinetic reasons much of its toxicity is likely to be mediated by its partially reduced forms, superoxide (O2-) and hydrogen peroxide (H2O2). Virtually all aerobic organisms contain enzymes devoted to eliminating these species from the cell: superoxide dismutase for O2-, and catalases and peroxidases for H2O2. This implies that O2- and H2O2 are formed somehow during aerobic metabolism and that, unless scavenged, they will harm the cell. Indeed, mutants that are unable to detoxify these oxygen species have difficulty growing in the presence of oxygen.
Yet the most fundamental questions about O2- and H2O2 toxicity remain unanswered: How do these species arise in the cell? What damage can they do? What do cells do to avoid or repair that damage? And when in nature does oxidative damage determine the fate of the cell?
Our laboratory pursues these questions primarily in E. coli, a model organism whose metabolism is well-understood and which is amenable to easy genetic manipulation. However, the mechanisms by which oxidants damage E. coli, and the strategies by which E. coli defends itself, are widely shared by other bacteria—and, indeed, by higher organisms.
How are superoxide and hydrogen peroxide formed inside cells? Mutant strains of E. coli that lack superoxide dismutase or catalases/peroxidases are very sick. Yet E. coli has no enzymes that produce O2- or H2O2 as deliberate products. So what is the source of the O2- and H2O2 that disable these mutants? We have found that some flavoenzymes inadvertently transfer electrons to dissolved oxygen when it bounces into their reduced cofactors. O2- is formed if one electron is transferred, and H2O2 is formed if two electrons are transferred. Not surprisingly, this behavior is particularly marked if the flavin is solvent-exposed, has a high electron density, and has a low reduction potential. Several respiratory dehydrogenases have these traits, and they generate significant O2- and H2O2 during respiration. However, genetic analyses show that the primary source(s) of oxidants inside E. coli lie outside the respiratory chain. The identification of these sources is currently an important goal of our lab.
What biomolecules does superoxide damage? Superoxide and hydrogen peroxide are used as weapons throughout the biological world: microbes and plants secrete antibiotics which, when ingested by competitors, redox-cycle to generate crippling doses of intracellular O2- and H2O2, and mammals blast invasive bacteria with O2- and H2O2 that is generated by specialized enzymes on the phagosomal membranes of neutrophils and macrophages. Yet, despite the fact that 100,000+ articles that address superoxide can be found in PubMed, we still are trying to identify the biological molecules that it damages. Superoxide does not react directly with polypeptides, sugars, or nucleic acids, and it is unlikely to trigger lipid peroxidation because bacterial membranes lack polyunsaturated fatty acids. In the 1980s studies in E. coli revealed that O2- disables the TCA cycle and branched-chain biosynthetic pathways by destroying the iron-sulfur clusters of critical enzymes. We have recently determined that O2- also inactivates certain enzymes that use a single ferrous iron atom to bind their substrates. Superoxide oxidizes this iron atom, the oxidized iron falls out of the enzyme, and other metals--that do not provide activity--bind in its place. This family of enzymes includes members that catalyze a wide range of reaction types, and their inactivation blocks a variety of cell functions. These must not be the only toxic actions of O2-, however, since SOD-deficient E. coli exhibit metabolic defects that these injuries cannot explain. We are analyzing these phenotypes in order to pinpoint additional biomolecules that O2- damages.
What biomolecules does hydrogen peroxide damage? H2O2 oxidizes transition metals. This behavior has several consequences for cell health. First, like O2-, H2O2 directly damages the iron-sulfur clusters of key enzymes. Second, H2O2 also inactivates ferrous-iron enzymes. Third, H2O2 reacts with the intracellular pool of unincorporated ferrous iron and thereby generates hydroxyl radicals. The latter species react avidly with virtually all biomolecules—including DNA. This injury causes mutations, a fact that has led many scientists to postulate that in mammals chronic oxidative DNA damage is a root cause of carcinogenesis and, perhaps, aging. Finally, as a consequence of its reactions with iron, H2O2 generally disrupts iron homeostasis and metabolism. This makes it difficult for cells to deliver iron into new metalloproteins. We are working to illuminate details of these damage processes (see figure).
How does E. coli defend itself against oxidants? While superoxide dismutase, catalases, and peroxidases scavenge O2- and H2O2, they can never diminish the intracellular levels to zero. Therefore many additional defensive measures are taken. E. coli repairs the iron-sulfur clusters of damaged enzymes by re-reducing their clusters and replacing their lost iron atoms. If the rate of enzyme damage exceeds the repair capacity, E. coli goes further, replacing some of the damaged iron-sulfur enzymes--aconitase and fumarase—with isozymes that do not use iron and that are therefore resistant to oxidation. During periods of oxidative stress E. coli also synthesizes Dps, an iron-sequestering protein that minimizes the amount of unincorporated iron that is available to react with H2O2. This action substantially suppresses DNA damage. Since this action would leave iron-dependent enzymes without a cofactor metal, the cell simultaneously activates the import of manganese as a replacement metal. Each of these adjustments is necessary—if any one of them is blocked, H2O2-stressed cells do not survive. Genetic analyses have identified many other genes that are deliberately induced in response to O2- or H2O2. We are still working to identify their roles.
Why can't anaerobes grow aerobically? How do phagocytes kill bacteria? And other questions. . . . Oxidative stress determines the fate of bacteria in several situations. Obligate anaerobes cannot grow in the presence of oxygen, a feature that profoundly constrains their lifestyle but is not well understood. Most bacteria are overpowered by the oxidative burst of phagocytes—but a select few pathogens somehow tolerate it. We are hopeful that E. coli will provide insights into these problems, too. At this point we know that some of the enzymes that oxygen inactivates in obligate anaerobes are the same ones that superoxide damages in E. coli. We also know that some of the oxidative defenses that were identified in E. coli are essential to the success of invading pathogens. Thus the lessons from E. coli provide direction for investigations of oxidative stress in these other organisms as well.
Imlay, James A., Ramakrishnan Sethu, and Sanjay K. Rohaun. 2019. Evolutionary adaptations that enable enzymes to tolerate oxidative stress. Free Rad. Biol. Med. 140: 4-13.
Lu, Zheng, and James A. Imlay. 2019. A conserved motif liganding the [4Fe-4S] cluster in [4Fe-4S] fumarases prevents irreversible inactivation of the enzyme during hydrogen peroxide stress. Redox Biology 26: 101296.
Korshunov, Sergey, Karin R. Chonoles Imlay, and James A. Imlay. 2019. Cystine import is a valuable but risky process whose hazards Escherichia coli minimizes by inducing a cysteine exporter. Mol. Microbiol. 113: 22-39. Khademian, M., and James A. Imlay. 2020. Do reactive oxygen species or does oxygen itself confer obligate anaerobiosis? The case of Bacteroides thetaiotaomicron. Mol. Microbiol., epub ahead of print.
Zhou, Y., and James A. Imlay. 2020. Escherichia coli K-12 lacks a high-affinity assimilatory cysteine importer. mBio 11:e01073-20.
Sen, A., and James A. Imlay. 2020. During oxidative stress the Clp proteins of Escherichia coli ensure that iron pools remain sufficient to reactivate oxidized metalloenzymes. J. Bacteriol., in press.