Host-Pathogen Interactions, Microbial Physiology, Regulation of Gene Expression, Sensory Processing, Signal Transduction
Disease Research Interests
Exploring the intersection of bacteria and the physical world
In the Sanfilippo lab, we use an interdisciplinary strategy that combines approaches from biology, chemistry, physics, and engineering to probe how physical forces impact bacterial cells.
Bacteria sense shear flow and alter gene expression using rheosensing
Using a novel microfluidic-based transcriptomic approach, we were the first to discover that bacterial cells actively sense and respond to flow speed, through a process we named rheosensing (as rheo- is Greek for flow) (Sanfilippo et al, 2019, Nature Microbiology). A major focus of our research group is to discover the molecular and biophysical mechanisms that control rheosensing.
Using microfluidics to create realistic bacterial environments
Over the past 100 years, scientists have studied bacteria in simplified, static conditions. However, bacteria exist in complex, dynamic conditions in nature. Fluid flow is a fundamental driver of dynamic cellular systems, exemplified by the human urinary tract and bloodstream. In the Sanfilippo lab, we engineer custom microfluidic devices with a variety of heights, widths, and channel geometries to experimentally model realistic bacterial environments.
B.S. (Genetics), University of Wisconsin, 2011
Ph.D. (Microbiology), Indiana University, 2016
Post-doc, Princeton University, 2016-2020
Additional Campus Affiliations
Assistant Professor, Biochemistry
Sanfilippo, J.E., Lorestani, A, Koch, M.D., Bratton, B.P., Siryaporn, A., Stone, H.A., and Gitai, Z. Microfluidic-based transcriptomics reveal force-independent bacterial rheosensing. Nature Microbiology (2019).
Sanfilippo, J.E., Garczarek, L., Partensky, F. and Kehoe, D.M. Chromatic Acclimation in Cyanobacteria: A Diverse and Widespread Process for Optimizing Photosynthesis. Annual Review of Microbiology (2019).
Sanfilippo, J.E., Nguyen, A. A., Partensky, F., Karty, J. A., Strnat, J.A., Garczarek, L., Schluchter, W.M. and Kehoe, D.M. Interplay between differentially expressed enzymes contributes to light color acclimation in marine Synechococcus. PNAS (2019).
Sanfilippo, J.E., Nguyen, A. A., Karty, J. A., Shukla, A., Schluchter, W.M., Garczarek, L., Partensky, F., and Kehoe, D.M. Self-regulating genomic island encoding tandem regulators confers chromatic acclimation to marine Synechococcus. PNAS (2016).
Haney, A. M., Sanfilippo, J. E., Garczarek, L., Partensky, F., & Kehoe, D. M. (2022). Multiple Photolyases Protect the Marine Cyanobacterium Synechococcus from Ultraviolet Radiation. mBio, 13(4). https://doi.org/10.1128/mbio.01511-22
Yang, J. Q., Sanfilippo, J. E., Abbasi, N., Gitai, Z., Bassler, B. L., & Stone, H. A. (2021). Evidence for biosurfactant-induced flow in corners and bacterial spreading in unsaturated porous media. Proceedings of the National Academy of Sciences of the United States of America, 118(38), [e2111060118]. https://doi.org/10.1073/pnas.2111060118
Carrigee, L. A., Mahmoud, R. M., Sanfilippo, J. E., Frick, J. P., Strnat, J. A., Karty, J. A., Chen, B., Kehoe, D. M., & Schluchter, W. M. (2020). CpeY is a phycoerythrobilin lyase for cysteine 82 of the phycoerythrin I α-subunit in marine Synechococcus. Biochimica et Biophysica Acta - Bioenergetics, 1861(8), . https://doi.org/10.1016/j.bbabio.2020.148215
Sanfilippo, J. E., Garczarek, L., Partensky, F., & Kehoe, D. M. (2019). Chromatic acclimation in cyanobacteria: A diverse and widespread process for optimizing photosynthesis. Annual review of microbiology, 73, 407-433. https://doi.org/10.1146/annurev-micro-020518-115738
Sanfilippo, J. E., Nguyen, A. A., Garczarek, L., Karty, J. A., Pokhrel, S., Strnat, J. A., Partensky, F., Schluchter, W. M., & Kehoe, D. M. (2019). Interplay between differentially expressed enzymes contributes to light color acclimation in marine Synechococcus. Proceedings of the National Academy of Sciences of the United States of America, 116(13), 6457-6462. https://doi.org/10.1073/pnas.1810491116