Host-Pathogen Interactions, Virology
Disease Research Interests
Immune evasion strategies of viruses; molecular disinfection of viruses; molecular tracking viruses in the environment
Viruses are one of the most abundant microorganisms on the planet, infecting every form of life from humans to bacteria. Mammalian viruses, many of which are zoonotic (i.e., transmitted to humans from other animals), are directly responsible for some of the worst diseases known to man (e.g., Ebola virus, HIV), and for common causes of morbidity and mortality around the world (e.g., norovirus, influenza virus).
Understanding, detecting and controlling viruses continue to be grand challenges in science because so little is known about the fundamental properties of viruses and their interactions with the environment, and because they vary greatly with respect to their genomes and replication cycles.
I am interested in asking fundamental questions about virology:
- What are the molecular properties of viruses that make them resistant or susceptible to disinfection in natural settings versus hospitals or water treatment plants?
- How do viruses evade the immune system to cause disease?
- How does the physical environment affect virus evolution and spread?
B.A. (Microbiology), Miami University, 1990
Ph.D. (Immunology and Molecular Pathogenesis), Emory University, 1996
Postdoctoral (Virology), Laboratory of Viral Diseases, National Institutes of Health, 1997-2001
Additional Campus Affiliations
Oh, C., Zhou, A., O’Brien, K., Schmidt IV, A. R., Shisler, J. L., Schmidt, A. R., Keefer, L., Brown, W. M., & Nguyen, T. H. (2023). Improved performance of nucleic acid-based assays for genetically diverse norovirus surveillance. medRxiv. https://doi.org/10.1101/2023.03.13.23286721
Storms, S. M., Shisler, J., Nguyen, T. H., Zuckermann, F. A., & Lowe, J. F. (2023). RT-LAMP as Diagnostic Tool for Influenza—A Virus Detection in Swine. Veterinary Sciences, 10(3), Article 220. https://doi.org/10.3390/vetsci10030220
Uprety, S., Sherchan, S. P., Narayanan, P., Dangol, B., Maggos, M., Celmer, A., Shisler, J., Amarasiri, M., Sano, D., & Nguyen, T. H. (2023). Microbial assessment of water, sanitation, and hygiene (WaSH) in temporary and permanent settlements two years after Nepal 2015 earthquake. Science of the Total Environment, 877, Article 162867. https://doi.org/10.1016/j.scitotenv.2023.162867
Usmani, M., Uprety, S., Bonham, N., Jamal, Y., Mao, Y., Sano, D., Shisler, J., Unnikrishnan, A., Nguyen, T. H., & Jutla, A. (2023). Assessment of pathogens in flood waters in coastal rural regions: Case study after Hurricane Michael and Florence. PloS one, 18(8 August), Article e0273757. https://doi.org/10.1371/journal.pone.0273757
Cong, W., Pike, A., Gonçalves, K., Shisler, J. L., & Mariñas, B. J. (Accepted/In press). Inactivation Kinetics and Replication Cycle Inhibition of Coxsackievirus B5 by Free Chlorine. Environmental Science and Technology. https://doi.org/10.1021/acs.est.2c09269