Department of Physics
1110 W. Green Street, MC-704
Urbana, IL 61801-3080
Paul Selvin earned a Ph.D. from the University of California, Berkeley, in 1990. Formally it was in physics, but in reality, it was in biophysics. The last experiment for his thesis, for example, was measuring the torsional rigidity of DNA by looking at a fluorophore intercalated in DNA, twisting around. A paper based on this experiment was published in Science (Selvin, 1992, Science) and later confirmed in a single molecule experiment (Bryant, Nature, 2003).
Professor Selvin did two sets of experiments during his postdoctoral training. One was developing a new type of fluorescence resonance energy transfer (FRET) based on lanthanides, called luminescence resonance energy transfer (LRET). He later used this technique to discover how a potassium ion channel, in part responsible for nerve conduction, responds to voltage (Cha, 1999, Nature; Posson, 2005, Nature). The second involved single molecule fluorescence—the first measurement of FRET between a single donor and single acceptor (Ha, 1996, PNAS).
After joining the Department of Physics at the University of Illinois in 1997, his interest has turned to molecular motors—proteins whose job is moving cargoes around the cell. For example, chromosomes are bound to molecular motors (kinesin and dynein) and under their power, are moved into the two halves of a cell during cell division. Selvin’s task was to determine how the motors moved. They were known to be dimeric, with two “feet,” held together by a head that consumed ATP (the universal food of the cell) and a stalk that bound to the cargo. It was also known that for each ATP burned up, these proteins moved between 8 and 37 nm, depending on the individual motor.
But did they move in a walking-type motion (also called “hand-over-hand”), or did they move as an inchworm does? A walking-type motion would move the back foot twice the center-of-mass distance, with the front foot not translating at all; an inchworm-type motion would move all parts equally—equal to the center of mass. The problem was that this distance for the walking motion was only 74 nm for the biggest motor, and 16 nm for the smallest motor—or 37 nm and 8 nm for an inchworm motion—about 30 times smaller than the diffraction limit of light, arguably the smallest distance that can be resolved with visible light.
The trick to get around this problem was to realize that a single molecule’s position can be located arbitrarily well. It’s much like a mountain peak, which can be located to within a few yards, even though the mountain itself may be a a mile wide. Similarly, a single molecule’s center can be determined to be its width (~250 nm), divided by the square root of the numbers of photons collected (see left diagram). Selvin and his group managed to collect 10,000 photons in 0.5 s, meaning that the fluorophore’s center could be determined to ±1.25 nm (250/√104). With some clever chemistry, the single fluorophore could last several minutes, meaning that Selvin could watch the fluorescently-labeled molecular motor take several steps (Yildiz, 2003, Science). He called the technique FIONA—Fluorescence Imaging with One-Nanometer Accuracy.
The first measurement was performed in vitro to control the amount of ATP that the motor “ate,” thereby slowing it down so each individual step could be seen. With FIONA, Selvin found that from the biggest motor, Myosin V, (Yildiz, 2003, Science) to the smallest motor, kinesin, (Yildiz, 2004, Science), to one in between (Myosin VI), (Yildiz, 2004, J.Biol.Chem.), the motors all moved in a hand-over-hand, or walking, fashion. This discovery won the award as one of the ten most significant advances in science for 2003, as judged by Science magazine. It also won for Selvin’s student, Ahmet Yildiz, Science’s grand prize (a world-wide competition) for the most noteworthy Ph.D thesis.
Selvin then looked in vivo, i.e., inside a cell (Kural, 2005, Science). Here the time resolution had to be improved to 1 msec—a 500-fold increase—because the ATP was now not under his control, and was at a fairly high concentration. The motion of individual kinesins, and individual dyneins, could be measured, and surprisingly, they could move cargo around more than 10 times faster than what had been measured in vitro!
Selvin is now combing FIONA with optical traps (holding particles with light) in vivo, to see how many motors are causing such large velocities. Selvin’s future plans call for measurements in situ, for example, in Planaria and in C. Elegans, to test how these motors act in “real life.”
B.S. University of Michigan, Ann Arbor, 1983 (Physics)
Ph.D. University of California-Berkeley, 1990 (Physics)
Awards and Honors
- The International Raymond and Beverly Sackler Prize in Biophysics, 2006
- Faculty Member of the Precision Proteomics Research Theme, Institute for Genomic Biology, Univesity of Illinois at Urbana-Champaign, 2005-present
- Fellow, American Physical Society, 2004
- Physics Sony Faculty Scholar, University of Illinois College of Engineering, 2004
- Michael & Kate Bárány Award for Young Investigators, Biophysical Society, 2004
- Biophysical Society Council, 2004
- Received National Science Foundation CAREER Award--a most prestigious honor to "support exceptionally promising college and university junior faculty who are committed to the integration of research and education" (quote from the NSF), 2000
- Cottrell Scholar, Research Corporation (fewer than 20 of these awards are made annually), 2000
- Xerox Award for Faculty Research, University of Illinois College of Engineering, 2000
- Beckman Fellow, Center for Advanced Studies, University of Illinois at Urbana-Champaign, Spring 2000
- Listed in Offices of Instructional Resources "Lists of Teachers Ranked as Excellent," for P140, Fall 1999, Fall 2001, for Phys 398bio, Spr 2002, Spr 2003
- Research Innovation Award, Research Corporation, 1999
- Dye development work featured in News section of Analytical Chemistry
- Fluorescence Young Investigator Award, Biophysical Society, 1999
- Muscle research featured in Biophotonics International magazine, May 1999
- AAAS Mass Media Science and Engineering Fellow, 1990
- Office of Technology Assessment Congressional Fellow Winner, 1990
- Member: Amer. Assoc. Adv. of Science; American Physical Society; Biophysical Soc.
Additional Campus Affiliations
Professor, Cell and Developmental Biology
Professor, Micro and Nanotechnology Lab
Department Affiliate, Chemistry
Deng, H., Konopka, C. J., Prabhu, S., Sarkar, S., Medina, N. G., Fayyaz, M., Arogundade, O. H., Vidana Gamage, H. E., Shahoei, S. H., Nall, D., Youn, Y., Dobrucka, I. T., Audu, C. O., Joshi, A., Melvin, W. J., Gallagher, K. A., Selvin, P. R., Nelson, E. R., Dobrucki, L. W., ... Smith, A. M. (2022). Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging In Vivo, Ex Vivo, and In Situ. ACS Nano, 16(2), 1999–2012. https://doi.org/10.1021/acsnano.1c07010
Han, Z., Vaidya, R. M., Arogundade, O. H., Ma, L., Zahid, M. U., Sarkar, S., Kuo, C. W., Selvin, P. R., & Smith, A. M. (Accepted/In press). Structural Design of Multidentate Copolymers as Compact Quantum Dot Coatings for Live-Cell Single-Particle Imaging. Chemistry of Materials. https://doi.org/10.1021/acs.chemmater.2c00498
Ren, P., Tiede, C., Fanning, S. W., Adams, T., Speirs, V., Nelson, E., Cheng, C., Moore, T. W., Greene, G. L., Tomlinson, D., & Selvin, P. R. (Accepted/In press). Labeling of a mutant estrogen receptor with an Affimer in a breast cancer cell line. Biophysical journal. https://doi.org/10.1016/j.bpj.2022.06.028
Shukla, S., Troitskaia, A., Swarna, N., Maity, B. K., Tjioe, M., Bookwalter, C. S., Trybus, K. M., Chemla, Y. R., & Selvin, P. R. (Accepted/In press). High-throughput force measurement of individual kinesin-1 motors during multi-motor transport. Nanoscale. https://doi.org/10.1039/d2nr01701f
Jain, S., Shukla, S., Yang, C., Zhang, M., Fatma, Z., Lingamaneni, M., Abesteh, S., Lane, S. T., Xiong, X., Wang, Y., Schroeder, C. M., Selvin, P. R., & Zhao, H. (2021). TALEN outperforms Cas9 in editing heterochromatin target sites. Nature communications, 12(1), . https://doi.org/10.1038/s41467-020-20672-5